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FertiCult™ Aspiration Medium

Cell culture medium for oocyte pick-up
OocyteHandling

FertiCult™ Aspiration medium is a ready to use formulation for oocyte pick-up and/or follicular flushing.

All the products in the FertiCult™ product range undergo strict quality control to comply with the following specifications:

pH 7.3-7.6
Osmolality 270-290mOsm/kg
Sterility Sterile
Endotoxin < 0.25EU/mL
Mouse embryo test ≥ 80% blastocysts after 96h incubation
Shelf life 18 months from date of produce

FertiCult™ Aspiration medium is CE marked as a Class 3 medical device according to the European Medical Device Directive.


Product order codes

ASPI020 5x 20ml FertiCult Aspiration medium
ASPI100 5x 100ml FertiCult Aspiration medium


The product composition can be found in the MSDS (see Resources). Additional information on some components is provided below:

Component Benefit
HEPES (18 mM)

HEPES stabilizes the pH under air (Clark and Swain 2014), therefore CO2 incubation is not required.

Heparin sodium (2.5 IU/ml)

Heparin is added to the media to avoid blood clot formation in the tubing during aspiration and/or flushing of oocytes in Assisted Reproductive Technologies.

Heparin is naturally present in the uterine, tubal and follicular fluid (Agostini 2006), (Yanagishita 1994).

Prospective randomized trial revealed that heparinized saline or culture medium (2 IU/ml) allow for successful follicle flushing (Biljan, et al. 1997).


Product literature


     Falah, KM.; Banna, H.; Aghaways, HA.; Zangana, RA.; , Using in-vitro maturation of immature oocytes retrieved from poor responder patients to improve pregnancy outcomes in Sulaimani government region, Iraq., European Scientific Journal (2014),Vol.10,No.33,pp.195-211


Literature concerning the components


     Agostini, Arian I. de., An unexpected role for anticoagulant heparin sulfate proteoglycans in reproduction., Swiss Med Wkly (2006),Vol.136,No.37-38,pp.349-352
     Biljan, M.M., Dean,N., Hemmings,R., Bissonnette,F., and Tan,S.L. , Prospective randomized trial of the effect of two flushing media on oocyte collection and fertilization rates after in vitro fertilization., Fertil. Steril. (1997),Vol.68,pp.1132-1134
     Clark, N.A. and Swain, J.E., Buffering systems in IVF., Culture media, Solutions, and Systems in Human ART, by P. Quinn (2014),pp.30-46
     Yanagishita, M., Proteoglycans and hyaluronan in female reproductive organs., EXS (1994),Vol.70,pp.179-90

Resources

Click on the links below for more information.