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Spermac Stain™

Sperm morphology stain

Assessment of sperm morphology is a standard procedure in semen analysis. The Spermac Stain™ method is simple yet perfectly adequate for the detailed investigation of morphology of all parts of the spermatozoa.
The stained smears are assessed under direct bright light and oil immersion (1000x).

Spermac Stain™ has been CE marked according to the specifications set forth in the European Medical Device Directive 98/79/EC.

We recommend to watch our demonstration video of the Spermac Stain kit:
https://www.youtube.com/watch?v=rvVt2LzoNmo

Product order codes

SPS050 Spermac Stain - 4 x 50mL
SPS250 Spermac Stain - 4 x 250mL

Interpretation of the results



Pictures from Professor Philip Chan (Loma Linda Univ. Gyn/Ob-IVF, USA)


Literature


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Zalata A.A., Morsy, H.K., Badawy Ael N., Elhanbly S., Mostafa T. , ACE gene insertion/deletion polymorphism seminal associations in infertile men., J Urol (2012),Vol.187,No.5,pp.1776-1780

Bencharif D., Amirat, L., Pascal O., Anton M., Schmitt E., Desherces S., Delhomme G., Langlois M.L., Barriere P., Larrat M., Tainturier D. , The advantages of combining low-density lipoproteins with glutamine for cryopreservation of canine semen., Reprod Domest Anim (2010),Vol.45,No.2,pp.189-200

El-Harouny M.A., Zalata A.A., Naser M.E., Abo El-Atta H.M., El-Shawaf I.M., Mostafa T., Long-term ofloxacin testicular toxicity: an experimental study., Andrologia (2010),Vol.42,No.2,pp.92-96

Lockyear K.M., Goodrowe K.L., Waddell W.T. and MacDonald S.E. , Comparison of different osmolalities and egg-yolk composition in processing media for the cryopreservation of red wolf (Canis rufus) sperm., Theriogenology (2009),Vol.71,No.3,pp.469-479

Lukanov T.H., Lichev D.I., Konova E.I., Emin A.I., Ayvazova N.P., Velkova A.V., Roussev R.G. , Flow cytometric measurement of sperm nuclear DNA fragmentation in infertile men with normal standard sperm parameters., Journal of Mens Health (2009),Vol.6,No.1,pp.50-55

Webb G.W., Dean M.M. , Effect of Centrifugation Technique on Post-storage Characteristics of Stallion Spermatozoa., Journal of Equine Veterinary Science (2009),Vol.29,No.9,pp.675-680

Cipak A., Stanic P., Duric K., Serdar T., Suchanek E. , Sperm morphology assessment according to WHO and strict criteria: method comparison and intra-laboratory variability., Biochemica Medica (2009),Vol.19,No.1,pp.87-94

Parapanov RN, Nusslé S., Crausaz M., Senn A., Hausser J., Vogel P., Testis size, sperm characteristics and testosterone concentrations in four species of shrews (Mammalia, Soricidae), Animal Reproduction Science (2009),Vol.114,pp.269-278

Zalata A.A., Hassan A.H., Nada H.A., Bragais F.M., Agarwal A., Mostafa T. , Follicle-stimulating hormone receptor polymorphism and seminal anti-Mullerian hormone in fertile and infertile men., Andrologia (2008),Vol.40,No.6,pp.392-397

Rota A., Magelli C., Panzani D., Camillo F., Effect of extender, centrifugation and removal of seminal plasma on cooled-preserved Amiata donkey spermatozoa, Theriogenology (2008),Vol.69,pp.176-185

Levitas E., Lunenfeld E., Weisz N., Friger M., Potashnik G., Relationship between age and semen parameters in men with normal sperm concentration: analysis of 6022 semen samples, Andrologia (2007),Vol.39,pp.45-50

Rota A., Milani C., Romagnoli S., Effect of post-thaw dilution with autologous prostatic fluid on dog semen motility and sperm acrosome status, Theriogenology (2007),Vol.67,pp.520-525

Levitas E., Lunenfeld E., Weisz N., Friger M. Har-Vardi I., Potashnik G., Relationship between sexual abstinence duration and the acrosome index in teratozoospermic semen: analysis of 1800 semen samples, Andrologia (2006),Vol.38,pp.110-112

Yakin K., Balaban B., Urman B., Is there a possible correlation between chromosomal variants and spermatogenesis?, International Journal of Urology (2005),Vol.12,pp.984-989

Biricik A., Guney I., Berkil H., Benkhalifa M., Kahraman S., A male (15;15) Robertsonian translocation case with 11 previous consecutive recurrent spontaneous abortions, Marmara Medical Journal (2004),Vol.17,No.1,pp.35-38

Salumets A., Effects of embryological parameters on the success of fresh and frozen embryo transfers, pHD dissertation. University of Helsinki, Tartu University Press, Finland (2003),Vol.1

Miller J.E., Smith T.T., The effect of intracytoplasmic sperm injection and semen parameters on blastocyst development in vitro, Human Reproduction (2001),Vol.16,No.5,pp.918-924

Svalander P., Jakobsson A-H., Forsberg A-S., Bengtsson A-C., Wikland M., The outcome of intracytoplasmic sperm injection is unrelated to 'strict criteria' sperm morphology, Human Reproduction (1996),Vol.11,No.5,pp.1019-1022

Resources

Click on the links below for more information.