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SpermMar™ IgG

Test for the detection of anti-sperm antibodies

SpermMar™ IgA and SpermMar™ IgG are rapid, easy-to-use tests for the detection of anti-sperm antibodies in semen and serum.
Anti sperm antibodies (ASA) are produced when the blood-testis barrier is disrupted by obstruction, infection or traumatism. Immunoglobulines of the IgG class are usually present in both serum and semen, and the IgG antibodies attach to the spermatozoa. ASA of the IgA class are secreted by the accessory sex glands and are found in semen only. ASA of the IgG class may be detected in seminal plasma, but there is usually no free IgA in seminal plasma. Anti sperm antibodies of the IgA class almost never occur without IgG antibodies.
In case more than 40% of motile spermatozoa present ASA of the IgG class, complementary testing for IgA antibodies is advisable.
The immunological factor reduces the probability of spontaneous conception, and this is particularly true when ASA of the IgA class are also present.

SpermMar™ IgG has an 18 month shelf life from date of produce.

We recommend to watch our demonstration video of the SpermMar Test IgG:
https://www.youtube.com/watch?v=pPpV_SHbKjI

Regulatory

Europe: CE-marked - USA: registered - Canada: Health Canada license
Brazil: registered - Australia: registered

Product order codes

SPMG_S SpermMar IgG singles - 50 tests
SPMG_C SpermMar IgG complete - 50 tests
SPMG_P Positive controls for SpermMar IgG
SPMG_N Negative controls for SpermMar IgG


Literature


     Al-Dujaily S.S., Chakir W.K., Hantoosh S.F., Direct Antisperm Antibody Examination of Infertile Men, Global Journal of Medical Research (2012),Vol.12,No.3

     Bohring C., Klepper L., Krause W., Localization of binding sites of naturally occurring antisperm antibodies on human spermatozoa by immunofluorescence, Andrologia (2004),Vol.36,pp.286-290

     Bohring C., Krause E., Habermann B., Krause W., Isolation and identification of sperm membrane antigens recognized by antisperm antibodies, and their possible role in immunological infertility disease, Molecular Human Reproduction (2001),Vol.7,No.2,pp.113-118

     Bohring C., Krause W., Characterization of Spermatozoa Surface Antigens by Antisperm Antibodies and its Influence on Acrosomal Exocytosis, American Journal of Reproductive Immunology (2003),Vol.50,pp.411-419

     Bohring C., Krause W., The characterization of human spermatozoa membrane proteins - surface antigens and immunological infertility, Electrophoresis (1999),Vol.20,pp.971-976

     Bohring C., Krause W., The intra- and inter-assay variation of the indirect mixed antiglobulin reaction test: is a quality control suitable?, Human Reproduction (1999),Vol.14,No.7,pp.1802-1805

     Bozhedomov V.A., Nikolaeva M.A., Ushakova I.V., Lipatova N.A., Bozhedomova G.E., Sukhikh G.T., Functional deficit of sperm and fertility impairment in men with antisperm antibodies , Journal of Reproductive Immunology (2015),Vol.112,pp.95-101

     Chavez-Badiola A., Drakeley A.J., Finney V., Sajjad Y., Lewis-Jones D.I., Necrospermia, antisperm antibodies, and vasectomy, Fertility and Sterility (2008),Vol.89,No.3,pp.723e5-e7

     Chiu W.W-C., Chamley L.W., Human seminal plasma prolactin-inducible protein is an immunoglobulin G-binding protein, Journal of Reproductive Immunology (2003),Vol.60,pp.97-111

     Depuydt C.E., Bosmans E., Zalata A., Schoonjans F., Comhaire F.H., The Relation Between Reactive Oxygen Species and Cytokines in Andrological Patients With or Without Male Accesory Gland Infection, Journal of Andrology (1996),Vol.17,No.6,pp.699-707

     Depuydt C.E., De Potter C.R., Zalata A., Baekelandt E., Bosmans E., Comhaire F.H., Levels of Hepatocyte Growth Factor/Scatter Factor (HGF/SF) in Seminal Plasma of Patients With Andrological Diseases, Journal of Andrology (1998),Vol.19,No.2,pp.175-182

     Garolla A., Pizzol D., Bertoldo A., De Toni L., Barzon L., Foresta C., Association, prevalence, and clearance of human papillomavirus and antisperm antibodies in infected semen samples from infertile patients, Fertility and Sterility (2013),Vol.99,No.1,pp.125-131

     Gatimel N., Moreau J., Isus F., Moinard N., Parinaud J., Leandri R.D., Anti-sperm antibodies detection by a modified MAR test: Towards a better definition of its indications, Reproductive biomedicine online (2018),Vol.37,No.6,pp.717-723

     Hadinedoushan H., Ghafourzadeh M., A survey of anti-sperm antibodies in infertile couples, Iranian Journal of Reproductive Medicine (2007),Vol.5,No.1,pp.39-40

     Hussein T.M., Elneily D., Eid A.A., Abou-ElKhier H., Assessment of antisperm antibodies in a sample of Egyptian patients with hepatitis C virus infection, Andrologia (2017),Vol.49,No.e12664

     Laudat A., Burc-Struxiano L., Is Absence of Spontaneous Agglutinates of Spermatozoa in Semen a Reliable Indicator of Non-Autosensization against Human Sperm Antigens?, Open Access Library Journal (2016),Vol.3,No.e2978

     Lotti F., Baldi E., Corona G., Lombardo F., Maseroli E., Degl'Innocenti S., Bartoli L., Maggi M., Epididymal more than testicular abnormalities are associated with the occurence of antisperm antibodies as evaluated by the MAR test, Human Reproduction (2018),Vol.33,No.8,pp.1417-1429

     Marconi M., Nowotny A., Pantke P., Diemer T., Weidner W., Antisperm antibodies detected by mixed agglutination reaction and immunobead test are not associated with chronic inflammation and infection of the seminal tract, Andrologia (2008),Vol.40,pp.227-234

     Marconi M., Pilatz A., Wagenlehner F., Diemer T., Weidner W., Are Antisperm Antibodies Really Associated with Proven Chronic Inflammatory and Infectious Diseases of the Male Reproductive Tract?, European Urology (2009),Vol.56,pp.708-715

     Ombelet W., Vandeput H., Janssen M., Cox A., Vossen C., Pollet H., Steeno O., Bosmans E., Treatment of male infertility due to sperm surface antibodies: IUI or IVF?, Human Reproduction (1997),Vol.12,No.6,pp.1165-1170

     Poppe K., Glinoer D., Tournaye H., Maniewski U., Haentjens P., Velkeniers B., Is systematic screening for thyroid disorders indicated in subfertile men?, European Journal of Endocrinology (2006),Vol.154,pp.363-366

     Rossato M., Galeazzi C., Ferigo M., Foresta C., Antisperm antibodies modify plasma membrane functional integrity and inhibit osmosensitive calcium influx in human sperm, Human Reproduction (2004),Vol.19,No.8,pp.1816-1820

     Tennakoon V., Weerasekera DS., Yasawardene SG., Successful pregnancy outcomes following in-vitro fertilization in infertile couples with high levels of antisperm antibodies, Sri Lanka Journal of Obstetrics and Gynaecology (2010),Vol.32,pp.47-48

     Tennakoon V., Yasawardene S.G., Weerasekera D.S., Possible risk factors for the formation of antisperm antibodies in a subfertile population, Sri Lanka Journal of Obstetrics and Gynaecology (2011),Vol.33,pp.12-19

     VerÓn G.L., Molina R.I., Tissera A.D., Estofan G.M., MarÍn-Briggiler C.I., Vazquez-Levin M.H., Incidence of Sperm Surface Autoantibodies and Relationship with Routine Semen Parameters and Sperm Kinematics, Am J Reprod Immunol (2016),Vol.76,pp.59-69


Background information


     Comhaire F., Vermeulen L., Human semen analysis, Human Reproduction Update (1995),Vol.1,No.4,pp.343-362

     Comhaire F.H., Mahmoud A.M.A., Depuydt C.E., Zalata A.A., Christophe A.B., Mechanisms and effect of male genital tract infection on sperm quality and fertilizing potential: the andrologist's viewpoint, Human Reproduction Update (1999),Vol.5,No.5,pp.393-398

     Djaladat H., Mehrsai A., Rezazade M., Djaladat Y., Pourmand G., Varicocele and Antisperm Antibody: Fact or Fiction?, Southern Medical Journal (2006),Vol.99,No.1,pp.44-48

     Francavilla F., Santucci R., Barbonetti A., Francavilla S., Naturally-occuring antisperm antibodies in men: interference with fertility and clinical implications. An update., Fronties in Bioscience (2007),Vol.12,No.May,pp.2890-2911

     Hinting A., Vermeulen L., Comhaire F., The indirect mixed antiglobulin reaction test using a commercially available kit for the detection of antisperm antibodies in serum, Fertility and Sterility (1988),Vol.49,No.6,pp.1039-1044

     Hossain A., Islam N., Schultz N., Pratt D., Madanes A., Evidence of a Relationship Between Age of the Infertile Patient and the Prevalence of Antisperm Antibody (ASA)., Fertility and Sterility (2008),Vol.84,No.S1,pp.S457

     Kamieniczna M., Domagała A., Kurpisz M., The frequency of antisperm antibodies in infertle couples - a Polish pilot study, Medical Science Monitor (2003),Vol.9,No.4,pp.194-201

     Kay D.J., Boettcher B., Comparison of the SpermMar Test with Currently Accepted Procedures for Detecting Human Sperm Antibodies, Reprod. Fertil. Dev. (1992),Vol.4,pp.175-181

     Kendirci M., Hellstrom W.J.G., Antisperm Antibodies and Varicocele., Southern Medical Journal (2006),Vol.99,No.1,pp.13-15

     Lähteenmäki A., In-vitro fertilization in the presence of antisperm antibodies detected by the mixed antiglobulin reaction (MAR) an the tray agglutination test (TAT), Human Reproduction (1993),Vol.8,No.1,pp.84-88

     Lombardo F., Gandini L., Dondero F., Lenzi A., Immunology and immunopathology of the male genital tract. Antisperm immunity in natural and assisted reproduction, Human Reproduction Update (2001),Vol.7,No.5,pp.450-456

     Lwaleed B., Greenfield R.S., Birch B.R., Cooper A.J., Does human semen contain a functional haemostatic system? A possible role for Tissue Factor Pathway Inhibitor in fertility through semen liquefaction, Thromb Haemost (2005),Vol.93,No.5,pp.847-852

     Mahmoud A., Comhaire F., DEBATE Antisperm antibodies: Use of the mixed agglutination reaction (MAR) test using latex beads, Human Reproduction (2000),Vol.15,No.2,pp.231-233

     Räsänen M., Saarikoski S., Penttilä I., Agrawel Y.P, A flow cytometric study on the effect of low dose cyclic prednisole treatment on sperm-bound antibody levels, Human Reproduction (1994),Vol.9,No.5,pp.889-893

     Räsänen M.L., Havatta O.L., Penttila I.M., Agrawal Y.P., Detection and quantitation of sperm-bound antibodies by flow cytometry of human semen , Journal of Andrology (1992),Vol.13,No.1,pp.55-64

     Rajah S.V., Parslow J.M., Howell R.J.S., Hendry W.F., Comparison of mixed antiglobulin reaction and direct immunobead test for detection of sperm-bound antibodies in subfertile males, Fertility and Sterility (1992),Vol.57,No.6,pp.1300-1303

     Verheyen G., Tournaye H., Laurier K., Devroey P., Van Steirteghem A., Auto-controlled study on in-vitro fertilization performance with 'antibody-free' spermatozoa selected by immunobead adsorption from semen of patients with anti-sperm antibodies, Human Reproduction (1994),Vol.9,No.6,pp.1119-1126

Resources

Click on the links below for more information.