Leucocyte staining method

Almost every semen sample contains cells other than spermatozoa. These may be polygonal cells from the genital tract, but mostly they are nucleated round cells. Round cells are either spermatogenetic precursor cells (spermatids, spermatocytes, sometimes spermatogonia) or white blood cells. Amongst the latter, lymphocytes are rather uncommon, and the majority are polymorphonuclear granulocytes, characterized by granules containing the enzyme peroxidase.
LeucoScreen™ differentiates round cells on the basis of their peroxidase content.

LeucoScreen™ has a 12 month shelf life from date of produce.

We recommend to watch our demonstration video of the LeucoScreen kit:


Europe: CE-marked - USA: registered - Canada: registered
Brazil: registered - Australia: registered

Product order codes

LEUCO LeucoScreen - 20mL


El-Taieb MAA, Herwig R, Nada E.A., Greilberger J., Marberger M., Oxidative stress and epididymal sperm transport, motility and morphological defects, European Journal of Obstetrics & Gynecology and Reproductive Biology (2009),Vol.144,pp.S199-S203

Khosrowbeygi A., Nosratollah Z., Seminal plasma levels of free 8-isoprostane and its relationship with sperm quality parameters, Indian Journal of Clinical Biochemistry (2008),Vol.23,No.1,pp.49-52

Prisant N., Escalier D., Soufir J-C., Morillon M., Schoevaert D., Misrahi M., Tachdjian G., Ultrastructural nuclear defects and increased chromosome aneuploidies in spermatozoa with elongated heads, Human Reproduction (2007),Vol.22,No.4,pp.1052-1059

Khosrowbeygi A., NosratollahZ., Fatty acid composition of human spermatozoa and seminal plasma levels of oxidative stress biomarkers in subfertile males, Prostaglandins, Leukotrienes And Essential Fatty Acids (2007),Vol.77,pp.117-121

Salsabili N., Ziaei A.M., Taheri M., Akbari F., Jalaie Sh., Impact of Sperm Collection Methods on Sperm Parameters in Spinal Cord Injured Men and Compared to Normal Controls in ICSI Program, Sexual Disability (2006),Vol.24,pp.141-149

Vujisic S., Lepej S.Z., Jerkovic L., Emedi I., Sokolic B., Antisperm Antibodies in Semen, Sera and Follicular Fluids of Infertile Patients: Relation to Reproductive Outcome after In Vitro Fertilization, American Journal of Reproductive Immunology (2005),Vol.54,pp.13-20

Yilmaz S., Koyaturk M., Kilic G., Alpak O., Aytoz A., Effects of leucocytospermia on semen parameters and outcomes of intracytoplasmic sperm injection, International Journal of Andrology (2005),Vol.28,pp.337-342

Zarghami N., Khosrowbeyhi A., Seminal plasma levels of 15-F2alpha-Isopropane, malodialdehyde and total homocysteine in normozoospermic and asthenozoospermic males, Indian Journal of Clinical Biochemistry (2005),Vol.20,No.2,pp.86-91

Johanisson E., Campana A., Luthi R., de Agostini A., Evaluation of 'round cells' in semen analysis: a comparative study, Human Reproduction Update (2000),Vol.6,No.4,pp.404-412

Griveau J.F., Grizard G., Boucher D., Le Lannou D., Influence of oxygen tension on function of isolated spermatozoa from ejaculates of oligozoospermic patients and normozoospermic fertile donors, Human Reproduction (1998),Vol.13,No.11,pp.3108-3113

Calogero A.E., Fishel S., Hall J., Ferrara E., Vicari E., Green S., Hunter A., Burrello N., Thornton S., D'Agata R., Correlation between intracellular cAMP content, kinematic parameters and hyperactivation of human spermatozoa after incubation with pentoxifylline, Human Reproduction (1998),Vol.13,No.4,pp.911-915

Zalata A.A., Christophe A.B., Depuydt C.E., Schoonjans F., Comhaire F.H., The fatty acid composition of phospholipids of spermatozoa from infertile patients, Molecular Human Reproduction (1998),Vol.4,No.2,pp.111-118

Thomas J., Fishel S.B., Hall J.A., Green S., Newton T.A., Thronton S.J., Increased polymorphonuclear granulocytes in seminal plasma in relation to sperm morphology, Human Reproduction (1997),Vol.12,No.1,pp.2418-2421

Ombelet W., Bosmans E., Janssen M., Cox A., Vlasselaer J., Gyselaers W., Vandeput H., Gielen J., Pollet H., Maes M., Steeno O., Kruger T., Semen parameters in a fertile versus subfertile population: a need for change in the interpretation of semen testing, Human Reproduction (1997),Vol.12,No.5,pp.987-993

Griveau J.F., Le Lannou D., Influence of oxygen tension on reactive oxygen species production and human sperm function, International Journal of Andrology (1997),Vol.20,pp.195-2000

Depuydt C.E., Bosmans E., Zalata A., Schoonjans F., Comhaire F., The Relation Between reactive Oxygen Species and Cytokines in Andrological Patients With or Without Male Accessory Gland Infection, Journal of Andrology (1996),Vol.17,No.6,pp.699-707

Comhaire F.H., Vermeulen L., Human semen analysis, Human Reproduction Update (1995),Vol.1,No.4,pp.343-362

Comhaire F., Depypere H., Millingos S., Statement on intra-uterine insemination, International Journal of Andrology (1995),Vol.18,No.S2,pp.76-77


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